KMID : 1195620130060040219
|
|
Clinical and Experimental Otorhinolaryngology 2013 Volume.6 No. 4 p.219 ~ p.225
|
|
Protective Role of Trimetazidine Against Neomycin-induced Hair Cell Damage in Zebrafish
|
|
Chang Ji-Won
Im Gi-Jung Chae Sung-Won Lee Seung-Hoon Kwon Soon-Young Jung Hak-Hyun Chung Ah-Young Park Hae-Chul Choi June
|
|
Abstract
|
|
|
Objectives: Trimetazidine (TMZ) is known to reduce the generation of oxygen-derived free radicals. The objective of the present study was to evaluate the effects of TMZ on neomycin-induced ototoxicity in transgenic zebrafish (Brn3C: EGFP).
Methods: Five-day, postfertilization zebrafish larvae were exposed to 125 ¥ìM neomycin and one of the following TMZ concentrations for 1 hour: 10 ¥ìM, 100 ¥ìM, 500 ¥ìM, 1,000 ¥ìM, 1,500 ¥ìM, or 2,000 ¥ìM. Hair cells within the neuromasts of the supraorbital (SO1 and SO2), otic (O1), and occipital (OC1) lateral lines were analyzed using fluorescence microscopy and confocal microscopy (n=10). Hair cell survival was calculated as a percentage of hair cells in the control group that were not exposed to neomycin. Ultrastructural changes were evaluated using scanning electron microscopy.
Results: TMZ protected against neomycin-induced hair cell loss in the neuromasts (TMZ 1,000 ¥ìM, 11.2¡¾0.4 cells; 125 ¥ìM neomycin only, 4.2¡¾0.5 cells; n=10; P<0.05) and decreased the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) reaction. In the ultrastructural analysis, structures of mitochondria and hair cells within the neuromasts were preserved in zebrafish exposed to 125 ¥ìM neomycin and 1,000 ¥ìM TMZ.
Conclusion: TMZ attenuated neomycin-induced hair cell loss in zebrafish. The results of this study suggest that neomycin induces apoptosis, and that apoptotic cell death can be prevented by treatment with tremetazidine.
|
|
KEYWORD
|
|
Trimetazidine, Neomycin, Ototoxicity, Zebrafish
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|